Project titles for:
Project titles for: 2008-10
- In silico analysis of Larger subunit of AGPase in Rice and Maize
- In silico Analysis of mutated in Larger subunit of AGPase in Maize
- Structural Comparison of Betaine Aldehyde Dehydrogenase-1 of Barley and Rice
- Analysis of Gene Expression Database of Skin Cancer
- Evaluation of Sequence Alignment Tools
- Phylogenetic analysis of Drb 3.2 gene
- Modelling of Smaller subunit of AGPase in Rice and Maize
- Prediction of Protein structure from sequence
- Development of Clinical Information Database of domestic animals
- To identify distant relationship between flavoprotein superfamily
- Promoters comparison in bacteria
- Transport protein features analysis
- Genome deciphering and comparative genomics of Solanaceous genome
- Computational approach in deciphering effect of gene alteration in Arabidopsis Thaliana
- Simulation of conformational changes in protein
Basmati rice constitute a small but special group, with superior aroma and grain quality. Fragrance is an important constituent for high-quality rice varieties. Rice (Oryza sativa) possesses two genes encoding betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) isozymes (BADH1) and (BADH2). The gene for BADH1 is located on chromosome four whilst the gene for BADH2 is located on chromosome eight. Recently, an eight base pair deletion and three SNPs in exon 7 of the gene encoding betaine aldehyde dehydrogenase 2 (BADH2) on chromosome 8 resulting in a frame shift that generate a premature stop codon that presumably disables the BADH2 enzyme of Oryza sativa. This mutant BADH2 allele (badh2) codes for a substantially truncated BADH2 enzyme, which is responsible for the elevated level of 2-actyl-1- pyrroline (2AP) which gives fragrant rice varieties Jasmine and Basmati their characteristics aroma.Due to great importance of BADH in rice, it has also been investigated in other cereals also. Barley is another imp crop which contains Badh1 and Badh2.BADH is an important enzyme in barley that catalyzes the last step in the synthesis of glycine betaine, a compatible solute accumulated by many plants under various abiotic stresses The accumulation of betaine and the induction of betaine aldehyde dehydrogenase were analyzed in salt-stressed barley leaves. Hence In Silico investigations has been done to compare barley BADH1 which is homologue of BADH2 with that of rice. Both BADH contains 505 Amino Acids and model was built using three programs: Modeller, Schrodingerand Swiss Model.Than comparisons of these models were done using various tools like MAMMOTH,GANGSTA+,ClustalW etc.