Project titles for:
Project titles for: 2008-10
- In silico analysis of Larger subunit of AGPase in Rice and Maize
- In silico Analysis of mutated in Larger subunit of AGPase in Maize
- Structural Comparison of Betaine Aldehyde Dehydrogenase-1 of Barley and Rice
- Analysis of Gene Expression Database of Skin Cancer
- Evaluation of Sequence Alignment Tools
- Phylogenetic analysis of Drb 3.2 gene
- Modelling of Smaller subunit of AGPase in Rice and Maize
- Prediction of Protein structure from sequence
- Development of Clinical Information Database of domestic animals
- To identify distant relationship between flavoprotein superfamily
- Promoters comparison in bacteria
- Transport protein features analysis
- Genome deciphering and comparative genomics of Solanaceous genome
- Computational approach in deciphering effect of gene alteration in Arabidopsis Thaliana
- Simulation of conformational changes in protein
The BoLA –DRB3.2 locus is highly polymorphic. More than 30 different alleles have been reported. The BoLA-DRB3.2 alleles potentially affect many traits related to immunity, SCC, and mastitis incidence. Bovine lymphocyte antigen (BoLA) is classified into four regions namely class IIb, class IIa, class III and class I from centromere to telomere direction. The class IIa region has DQ and DR as major loci. There are at least three DRB genes i.e.DRB1, DRB2, and DRB3.Class IIa region genes encode heterodimeric glycoproteins, which are composed of α and β chains, and expressed on the surface of antigen-presenting cells. These molecules bind processed peptides of exogenous foreign antigens and present them to specific T helper cells. The extensive structural polymorphism of the class II molecules is considered to be responsible for differences among individuals in the immune response to different infectious agents. Expression of MHC gene is, thus an essential component of studies on immune response and resistance/susceptibility to diseases. An analysis of MHC polymorphism is of interest because association have been found between MHC and various diseases in bovines.DRB3.2 gene have found to be associated with SCC(somatic cell count) and mastitis resistance in cattle. Gene DRB3.2 is taken from different species if bovines like; Bos taurus,Ovis aries,Merino,Bubalus bubalis,Capra hircus,Rupicapra,along with the species of Sus,Macaca and humans for better comparison. When the phylogenetic analysis of DRB3.2 gene from all these species is done, it is found that the gene is most distant in the species of Macaca, whereas it is least distant in the species of Bos and Bubalus. Trees were constructed from aligned sequences by distance methods and character-state methods. The distance methods used a difference matrix (or distance matrix) containing all the pairwise distances between all sequences. Distance methods include UPGMA, Neighbor Joining methods and Fitch Margoliash methods. Character state methods keep track of the amino acid or the nucleotide at a given site in a sequence. It includes Maximum Parsimony and Maximum Likelihood methods. The character state methods tend to be more computer intensive than distance methods. Present investigations showed that the distance between node 23 and most distant “Macaca mulatta” is 0.222010.Also distance, according to parsimony method between common node 1 and most distant species of “Macaca mulatta” is 0.374470.Distance between node 1 and “Bos taurus” species is 0.048130. In maximum likelihood method the similar sequences came under single node which shows that this method is good for divergent sequences.