Project titles for:
Project titles for: 2005-07
- Virtual Screening for Inhibitors against NS3 protease of dengue virus
- Virtual Screening of Tuberculosis Inhibitors
- Determination of Sequence Homology in Promoter region of drought and salt responsive genes in rice and/or cereals
- Designing of Nanobody against Dandruff which works under harsh conditions
- In silico Designing of Clinically Robust VHH antibody Against Rotavirus
- Phylogenetic Analysis of Bluetongue Virus using VP2 and VP7 genes
- Phylogenetic Analysis of Rotavirus using VP4 and VP7 gene segments
- Pharmacophore based Screening of HIV I Reverse Transcriptase Inhibitors
- Rice Genome Search for BAD -2 Homologous genes
- Putative functional annotation of genes near BAD-2 locus of rice chromosome 8
- Bioinformatics Web Tools
Malassezia furfur is an anthropophilic fungus that belongs to the physiological skin flora. It infects the human scalp by secreting protein Malf1. Mutated nanobody sequence reduces the Malf1 susceptibility that can neutralize Malassezia furfur The query sequence purposed by Dolk (2004) was taken as model sequence and was put as the query in BLAST-P. Multiple sequence alignment was done by clustalW and the most similar sequence with the already existing nanobody sequence was chosen. Then VHH model was generated with the approach of homology modeling. The selected nanobody sequence revealed the potential mutation site. Mutation of position 44 may resulted in dramatic increase in the structural property of VHHs that are functional in application and could be used in shampoo for the prevention of dandruff (referred to Dolk et al 2004). The resultant nanobody shows that in silico stabilization can contribute to functional improvement of VHH which in the case might lead to an economically feasible product for neutralization of Malassezia furfur for people in developing countries.