Project titles for:
Project titles for: 2005-07
- Virtual Screening for Inhibitors against NS3 protease of dengue virus
- Virtual Screening of Tuberculosis Inhibitors
- Determination of Sequence Homology in Promoter region of drought and salt responsive genes in rice and/or cereals
- Designing of Nanobody against Dandruff which works under harsh conditions
- In silico Designing of Clinically Robust VHH antibody Against Rotavirus
- Phylogenetic Analysis of Bluetongue Virus using VP2 and VP7 genes
- Phylogenetic Analysis of Rotavirus using VP4 and VP7 gene segments
- Pharmacophore based Screening of HIV I Reverse Transcriptase Inhibitors
- Rice Genome Search for BAD -2 Homologous genes
- Putative functional annotation of genes near BAD-2 locus of rice chromosome 8
- Bioinformatics Web Tools
VHH antibodies or nanobodies are the smallest antigen binding fragments of naturally occurring heavy chain antibodies that have been evolved eventually in the absence of light chain. The nanobodies comprise one of the smallest available intact antigen binding fragments (about~15KDa, 118-186 residues). These VHH are engineered from the HCAbs specifically present in camilidae family. The Rota virus susceptibility can be reduced if camelid single domain Ab can neutralize this antigen. Llama VHH1 (Dolk, 2004) antibody sequence revealed the potential mutation sites. Mutation of position 27 resulted in dramatic increase in the structural property of VHHs that have high utility in various applications and could be used as therapy against the Rota virus. Multiple sequence alignment was done and VHH model for Camel was generated with the help of various softwares JPred, stabilization studies can contribute to functional improvement of VHH. If wet lab experiments agree to the results of this study, it might lead to an economically feasible product for neutralization of Rota virus for people in developing countries.