Project titles for:
Project titles for: 2005-07
- Virtual Screening for Inhibitors against NS3 protease of dengue virus
- Virtual Screening of Tuberculosis Inhibitors
- Determination of Sequence Homology in Promoter region of drought and salt responsive genes in rice and/or cereals
- Designing of Nanobody against Dandruff which works under harsh conditions
- In silico Designing of Clinically Robust VHH antibody Against Rotavirus
- Phylogenetic Analysis of Bluetongue Virus using VP2 and VP7 genes
- Phylogenetic Analysis of Rotavirus using VP4 and VP7 gene segments
- Pharmacophore based Screening of HIV I Reverse Transcriptase Inhibitors
- Rice Genome Search for BAD -2 Homologous genes
- Putative functional annotation of genes near BAD-2 locus of rice chromosome 8
- Bioinformatics Web Tools
The BT viruses (BTV) constitute the type-species of the genus Orbivirus within the family Reoviridae. All analysis was performed using PHYLIP program. Nucleotide Sequences of VP2 and VP7 genes of bluetongue virus were selected in FASTA format from primary nucleotide sequence database, National Centre for Biotechnology Information (NCBI). Homology amongst these sequences was found using LALIGN program which compares two DNA sequences for local similarity and shows the local sequence alignment. The sequences left are subjected to multiple alignment by CLUSTAL X method. Trees were constructed from aligned sequences by distance methods and character-state methods. The distance methods used a difference matrix (or distance matrix) containing all the pairwise distances between all sequences. Distance methods include UPGMA, Neighbor Joining methods and Fitch Margoliash methods. Character state methods keep track of the amino acid or the nucleotide at a given site in a sequence. It includes Maximum Parsimony and Maximum Likelihood methods. In maximum likelihood method the similar sequences came under single node which shows that this method is good for divergent sequences.From above analysis it is concluded that in VP2 gene, Bluetongue virus VP5 gene for outer capsid protein is most evolved and it’s distance from the node is 2.09815 which is almost same for all distance based methods used in this project while it’s likelihood distance is 2.24222 and in VP7 gene, Mus musculus Bluetongue virus VP7-specific antibody is most evolved and it’s distance from the node is 0.28651 which is same for all distance methods while it’s likelihood distance is 1.86457.