Project titles for:
Project titles for: 2005-07
- Virtual Screening for Inhibitors against NS3 protease of dengue virus
- Virtual Screening of Tuberculosis Inhibitors
- Determination of Sequence Homology in Promoter region of drought and salt responsive genes in rice and/or cereals
- Designing of Nanobody against Dandruff which works under harsh conditions
- In silico Designing of Clinically Robust VHH antibody Against Rotavirus
- Phylogenetic Analysis of Bluetongue Virus using VP2 and VP7 genes
- Phylogenetic Analysis of Rotavirus using VP4 and VP7 gene segments
- Pharmacophore based Screening of HIV I Reverse Transcriptase Inhibitors
- Rice Genome Search for BAD -2 Homologous genes
- Putative functional annotation of genes near BAD-2 locus of rice chromosome 8
- Bioinformatics Web Tools
Rotavirus, a member of the Reoviridae family, is a casual agent of acute viral gastroenteritis in the young onces of a wide range of avain and mammalian species. Rotavirus is a non-enveloped virus with a double-stranded RNA (dsRNA) genome of 11 segments enclosed in a triple- layered protein capsid. VP4 and VP7 genes are the parts of 11 segmented genome .They synthesize the viral protein. Present study aimed at phylogenetic analysis of VP4 Aand VP7 genes. Molecular phylogeny was based on the comprision of DNA sequences of both the genes. Datasets comprising of 48 VP4 nucleotide sequences and 143 VP7 nucleotide sequences had been collected from NCBI database. As phylogenetic analysis can only be performed with aligned sequences, Clustal X was used for multiple sequence alignment. PHYLIP is the most comprehensive and widely used phylogeny package. DNADIST and Neighbor programe were used for generating distance matrix and tree drawing respectively. Phylograms and cladograms have been drawn using Treeview programme. Among VP4 genes, Rotavirus A clone O4 VP4 protein gene (gi|93138720|gb|DQ478585.1|) came out to be the earliest evolved. Rotavirus A clone B17 VP4 protein gene (gi|93138724|gb|DQ478587.1|) came out to be the most evolved. Among VP7 genes, Human rotavirus A isolate NIV931072 VP7 gene (gi|114152678|gb|DQ875601.1|) came out to be the earliest evolved. Bovine group B rotavirus outer capsid protein VP7 gene (gi|33327725|gb|AF529214.1|) came out to be the most evolved.